Aliases: DII4; IDA5; ACT1; Actin

Structure: Inner Arm Dynein; Mating; Actin Cytoskeleton

Human Homolog: multiple

V6 Gene Name & Defline: IDA5 | Actin, flagellar inner arm dynein subunit

Comments:
• Actin encoded by the IDA5 locus gi:34581636, (Kato-Minoura et al. Kamiya 1997 PMID 9151671)
• flagellar inner arm dynein subunit
• present in Inner Arm Dynein species a b c d e g and some minor species

Synonyms: NSG4; ACT1; DII4; g14567.t1

Locus: Cre13.g603700 | 4532_13_72005

CLIP Library: Cre13.g603700

V6 Transcripts:   Cre13.g603700_4532.1   

V5.6 Gene Name & Defline: DII4 | Actin, flagellar inner arm dynein subunit

Description: Actin encoded by the IDA5 locus [gi:34581636, PMID: 9151671]; flagellar inner arm dynein subunit; present in Inner Arm Dynein species a b c d e g and some minor species

Synonyms: Cre13.g603700.t1.1; g14567.t1; ACT1; NSG4

CLIP Library: Cre13.g603700

Phytozome Gene: Cre13.g603700

CrFP Total Peptides (Pazour et al. Witman 2005) = 19 | 2 Axonemal Peptides | 2 M+M Peptides | 10 KCl Peptides | 5 Tergitol Peptides

Minus Mating Type Peptides (Sakato-Antoku and King 2022) = CC124 Vegatative M&M: 33.52 | CC124 Vegatative Axonemes: 30.79 | CC124 Gamete M&M: 33.99 | CC124 Gamete Axonemes: 31.92 (Normalized Total Spectra)

Plus Mating Type Peptides (Sakato-Antoku and King 2022) = CC125 Vegatative M&M: 25.47 | CC125 Vegatative Axonemes: 33.04 | CC125 Gamete M&M: 30.36 | CC125 Gamete Axonemes: 28.99 (Normalized Total Spectra)

Wang Peptides (Wang et al. Pan 2017) = 7 (enriched 1.16 fold by ciliary shortening)

Jordan Peptides (Jordan et al. Pigino 2018) = Wild Type M+M: 12 | Dhc1b-3 M+M: 13 | Ift139-2 M+M: 12 (Total Unique Peptides)

Picariello Peptides (Picariello et al. Witman 2019) = Wild Type M+M: 15 | Ift140 ΔN-terminal WD repeat M+M: 16 (Total Unique Peptides)

Zhao Central Apparatus Project (Zhao et al. Witman 2019) = Wild Type Axo: 92 | Pf18 Axo: 94 (Total Unique Peptides, summed from two biological replicates for each. Proteins found only in this dataset needed 10 or more peptides to be included)

Dai Central Apparatus Project (Dai et al. Bui 2019) = Wild Type Axo: 12.67 | Pf6 Axo: 14.67 | Pf16 Axo: 12.67 | Cpc1 Axo: 10.00 | Fap52 Axo: 15.00

Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 0.98 | at 10 min: 1.13 | at 30 min: 1.90 | at 60 min: 2.70 (for reference tubulins are ~8 at 30 min)

Fold Upregulated Post Deflagellation by RNA seq (Wu et al. Ou 2022) at 15 min: 0.34 (p=9.0e-1) | at 30 min: 1.22 (p=3.2e-1) | at 60 min: 1.98 (p=1.8e-2) | at 90 min: 1.87 (p=2.7e-2) (for reference tubulins are ~3 at 30 min)

Fold Upregulated Post Deflagellation by RIBO seq (Wu et al. Ou 2022) at 15 min: 0.67 (p=1.0e+0) | at 30 min: 1.83 (p=5.7e-3) | at 60 min: 2.64 (p=1.1e-7) | at 90 min: 2.50 (p=1.5e-7) (for reference tubulins are ~3 at 30 min)

Translational Efficiency (the ratio of normalized RIBO-Seq and RNA-Seq reads per transcript, Wu et al. Ou 2022) at 15 min: 0.32 | at 30 min: 0.61 | at 60 min: 0.66 | at 90 min: 0.63 (for reference tubulins are ~-0.3 at 30 min)

Importance of XAP5 for expression (Li et al. Hu 2018)(PNAS Editors 2021): Wild Type: FPKM | xap5 Mutant: FPKM | Fold Change (for reference tubulins are ~-2)